ASSOCIAtIOnS Between pOLymOrphISmS In the LeptIn gene AnD mILK prODuCtIOn trAItS In pInzgAu

1 Slovak univ. of Agriculture in nitra, Dept. of Animal genetics and Breeding Biology, tr. A. hlinku 2, 949 76 nitra, Slovak republic, e-mail: [email protected] AbStrAct The aim of this study was to evaluate the relationship between the polymorphism in the leptin gene and milk production traits in pinzgau and Slovak Spotted cattle. A total of 85 pinzgau and 110 Slovak Spotted cows were used to investigate how leptin gene polymorphisms affect milk production traits. The polymorphism of leptin gene in intron 2 on bovine chromosome 4 was genotyped by using the polymerase chain restriction fragment length polymorphism. A strategy employing pCr was used to amplify a 422 bp product from DnA samples. Digestion of pCr products with restriction enzyme Sau3AI revealed two alleles: allele A gave two fragments,390 and 32 bp in length and allele B gave three fragments of 303, 88, 32 bp. The predominant allele was A with observed frequency 0.69 (pinzgau) and 0.83 (Slovak Spotted). In both populations were observed all three genotypes AA, AB and BB. results from the statistical analysis between Sau3AI polymorphism and milk production parameters – milk, protein and fat yield (kg) were not significant. The comparison of animals from both breeds with the same genotype shows significant effect in pinzgau cows with AA genotype on milk and protein yield (p ≤ 0.05).